Mémoire
| Résumé : | Trachycephalus resinifictrix and Osteocephalus taurinus are two closely related anuran species of the family Hylidae, yet distinguished by the adhesive nature of the skin secretion of T. resinifictrix. With an aim to explore the gene-encoded peptides and protein in their skins, this study performed expression analysis of de novo assembled transcriptomes of the dorsal skin of the two species. Screening for available bioactive peptides found protease inhibitors as the most abundant protein family in O. taurinus. Frog skin active peptides (FSAPs) are expressed with a high expression value in both transcriptomes, where they are the most abundant bioactive peptides in T. resinifictrix. Multiple transcripts encoding hormone/neuropeptide-like precursors are present in both transcriptomes but with a negligible expression level. Considering the IgGFcBP-like region and galectin as candidate bioadhesive peptides, screening results show similar expression levels in both the non-adhesive and adhesive frog species. Therefore, their incorporation in T. resinifictrix adhesive secretion cannot be proven by this study. Furthermore, phylogenetic reconstruction of FSAPs reveals the structural uniqueness of the newly found FSAPs of these two taxa, where they are nested with the other hyloid FSAPs but form a distinct clade. |
