Résumé : The specific binding of VIP to guinea pig brain membranes was tested by 1/ the ability of eight VIP and secretin analogs and fragments to inhibit the binding of 125I-VIP and 2/ the capacity of the same peptides to influcence basal and VIP-stimulated adenylate cyclase activities. Among all peptides tested, only VIP, secretin [Val5] secretin, and [Gln9, Asn15] secretin (5-27) were able to inhibit 125I-VIP binding. The adenylate cyclase activity was stimulated by VIP, secretin and [Val5] secretin. [Gln9, Asn15] secretin (5-27) although inactive per se was able to inhibit the VIP-stimulated adenylate cyclase activity competitively.