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Dissociation by methylamine of insulin release from glucose-induced electrical activity in isolated mouse islets of Langerhans.

par Lebrun, Philippe ;Atwater, I;Rosario, L M;Herchuelz, André ;Malaisse, Willy
Référence Metabolism, clinical and experimental, 34, 12, page (1122-1127)
Publication Publié, 1985-12

Article révisé par les pairs

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Résumé :

The effect of methylamine on electrical activity and simultaneously measured insulin release was investigated in single perifused islets of normal mice. Methylamine, (2 mmol/L or 6 mmol/L) failed to affect beta-cell input resistance and only caused a modest and transient inhibition of electrical activity of islets exposed to 11.1 mmol/L glucose. Methylamine (2 mmol/L) inhibited insulin release evoked by a five-minute rise in glucose concentration from 5.6 to 22.2 mmol/L, even when the glucose-induced electrical activity remained unaltered. Methylamine, at 2 or 5 mmol/L, partially inhibited insulin release but failed to affect the continuous electrical activity in islets exposed throughout to 22.2 mmol/L glucose. At 10 mmol/L, methylamine reduced both insulin release and electrical activity. These data reinforce the idea that the glucose-induced changes in beta-cell membrane potential represent an early event in the process of stimulus-secretion coupling and can be dissociated from the subsequent process of insulin release.