par Laurent, Eric 
;Mockel, J;Van Sande, Jacqueline ;Graff, Isabelle ;Dumont, Jacques Emile
Référence Molecular and cellular endocrinology, 52, 3, page (273-278)
Publication Publié, 1987-08
;Mockel, J;Van Sande, Jacqueline ;Graff, Isabelle ;Dumont, Jacques Emile Référence Molecular and cellular endocrinology, 52, 3, page (273-278)
Publication Publié, 1987-08
Article révisé par les pairs
| Résumé : | In human thyroid slices prelabeled with myo-[2-3H]inositol, thyrotropin (TSH, 3-30 mU/ml) stimulated IP3, IP2 and IP1 generation over a prolonged time course. The cAMP response was much more sensitive to TSH, peaking between 1 and 5 mU/ml. Forskolin (10(-5) M) and isoproterenol had no effect on basal IP levels, while carbamylcholine (10(-5) M, 10(-4) M) also increased IP accumulation. These data suggest that in the human thyroid, TSH activates a phospholipase C generating IP3 and diacylglycerol independently of the well-known adenylate cyclase stimulation. They validate in the human model a dual mode of action of the hormone previously proposed on the basis of indirect observations. |
