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Comparison of Trypanosoma cruzi lineages and levels of parasitic DNA in infected mothers and their newborns.

par Virreira Bermudez, Myrna ;Truyens, Carine ;Alonso-Vega, Cristina;Brutus, Laurent;Jijena, Juan;Torrico, F;Carlier, Yves ;Svoboda, Michal
Référence The American journal of tropical medicine and hygiene, 77, 1, page (102-106)
Publication Publié, 2007-07

Article révisé par les pairs

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Résumé :

To better understand the factors involved in maternal-fetal transmission of Trypanosoma cruzi, we compared DNA levels-obtained by use of quantitative real-time PCR and parasitic genotypes determined by PCR amplification followed by hybridization-in Bolivian mothers and their congenitally infected newborns. Mothers and their neonates displayed markedly different parasitic DNA levels, as most maternal estimated parasitemias (> 90%) were < 10 parasites/mL, whereas those of 76% of their newborns were > 1,000 parasites/mL. Comparison of T. cruzi TcII sublineages infecting mothers and newborns showed identity, without evidence of mixed infection in mothers or neonates. Analysis of minor variants of TcIId-genotyped parasites using sequence class probes hybridizing with hypervariable domains of kDNA minicircles showed discrepancies in half of mother/newborn pairs.