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Amniotic fluid is not useful for diagnosis of congenital Trypanosoma cruzi infection.

par Virreira Bermudez, Myrna ;Martinez, Sabrina;Alonso-Vega, Cristina;Torrico, F;Solano, Marco Antonio;Torrico, Mary Cruz;Parrado, Rudy;Truyens, Carine ;Carlier, Yves ;Svoboda, Michal
Référence The American journal of tropical medicine and hygiene, 75, 6, page (1082-1084)
Publication Publié, 2006-12

Article révisé par les pairs

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Résumé :

Although Trypanosoma cruzi can be transmitted transplacentally and induce congenital infection, no data are available about the presence of this parasite in human amniotic fluid. We examined 8, 19, and 4 amniotic fluid samples (collected at delivery or by aspiration of gastric content of neonates) from control uninfected mothers (M-B-), infected mothers delivering uninfected newborns (M+B-), and mothers of confirmed congenital cases (M+B+), respectively. Polymerase chain reaction (PCR), using nuclear and kinetoplastic DNA primers (Tcz1-Tcz2 and 121-122), were negative for all control M-B- samples, but positive for 5 of 19 M+B- and 2 of 4 M+B+ samples. To determine the number of parasites in the positive samples, real-time PCR using S35/S36 kinetoplastic DNA was performed. Only one M+B+ sample presented a high parasitic DNA amount, whereas the other six PCR-positive samples displayed traces of T. cruzi DNA. In conclusion, the release of parasites in amniotic fluid is probably a rare event that cannot be helpful for the routine diagnosis of congenital Chagas disease.