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A rapid test to monitor alloreactive responses in whole blood using real-time polymerase chain reaction.

par Zhou, Ling ;Toungouz Nevessignsky, Michel ;Donckier De Donceel, Vincent ;Andrien, Marc ;Troisi, Roberto;de Hemptinne, Bernard;Le Moine, Alain ;Dupont, Etienne ;Goldman, Michel ;Stordeur, Patrick
Référence Transplantation, 80, 3, page (410-413)
Publication Publié, 2005-08

Article révisé par les pairs

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Résumé :

Rapid, simple, and reliable assays to monitor allogeneic responses are essential for the safe development of novel protocols of tailored immunosuppression. Herein, we describe a real-time polymerase chain reaction method based on interleukin-2 and interferon-gamma mRNA quantification upon stimulation of whole blood with allogeneic T cell-depleted peripheral blood mononuclear cells. The technique requires only small blood volumes and results can be obtained within 48 hours. Data obtained in a liver transplant patient receiving a tolerance induction protocol based on the infusion of donor-type hematopoietic stem cells suggest that this rapid whole blood mixed lymphocyte reaction assay could be valuable for the monitoring of patients undergoing solid organ or hematopoietic stem cell transplantation.