par Schiavolin, Lionel 
;Lakhloufi, Dalila ;Botquin, Gwenaëlle ;Deneubourg, Geoffrey ;Bruyns, Corentin ;Steinmetz, Jenny ;Henrot, Charlotte ;Delforge, Valérie ;Smeesters, Pierre ;Botteaux, Anne
Référence Microbiology spectrum, 12, 10
Publication Publié, 2024-10-01
;Lakhloufi, Dalila ;Botquin, Gwenaëlle ;Deneubourg, Geoffrey ;Bruyns, Corentin ;Steinmetz, Jenny ;Henrot, Charlotte ;Delforge, Valérie ;Smeesters, Pierre ;Botteaux, Anne Référence Microbiology spectrum, 12, 10
Publication Publié, 2024-10-01
Article révisé par les pairs
| Résumé : | Streptococcus pyogenes or Group A Streptococcus (GAS) remains a significant infectious problem around the world, particularly in low- and middle-income settings. Moreover, a recent invasive GAS infection (iGAS) upsurge has been observed in high-income settings. However, to date, no vaccine is available. Finding a good vaccine antigen and understanding the role of virulence factors in GAS infections have been hampered, in part, by technical difficulties to transform the many different strains and generate knockout mutants. Using colE1-type plasmid as a suicide vector, we have set up a method allowing the generation of non-polar mutants of GAS in 3 days. |
