par Baltus, Elyane ;Hanocq Quertier, Jacqueline
Référence Gamete research, 8, 3, page (245-253)
Publication Publié, 1983
Article révisé par les pairs
Résumé : Xenopus laevis oocytes undergo maturation when they are injected with large quantities of crude ribosomes from various origins: X laevis full-grown or matured oocytes, Xenopus ovaries and embryos, Xenopus liver or mouse liver. All have the same efficiency, whatever their origin: they induce 50-90% maturation in the injected oocytes at about the same speed as progesterone treatment. The ribosomal preparations are inactive when injected into recipient oocytes pretreated with cholera toxin or cycloheximide. After dissociation of the ribosomes with 0.5 M KCl, the maturing activity was found to be associated with the high salt extract, but not with the subunits. Hypotheses concerning the mode of action of this ribosomal extract are discussed.