par Vos, Jean Michel H J.M.;Rommelaere, Jean
Référence Comptes rendus des séances de la Société de biologie et de ses filiales, 176, 3, page (395-401)
Publication Publié, 1982
Article révisé par les pairs
Résumé : The parvovirus Minute-Virus-of-Mice (MVM) was used to probe the DNA replication activities expressed by mouse fibroblasts. This sytem allowed us to study quantitatively the effect of UV-induced DNA lesions on the progression of DNA replication in vivo. MVM was UV-irradiated prior to infection. Pyrimidine dimers induced in the viral genome account for the reduced level of intracellular viral DNA synthesis, assuming that most of these lesions block viral DNA replication in unirradiated cells. The inhibition of damaged MVM DNA synthesis is less severe if the host cells themselves are irradiated prior to virus infection. This stimulation of viral DNA replication in pretreated cells might account for the UV-enhanced viral reactivation phenomenon, i.e., the increased survival of nuclear-replicating viruses propagated in cells preexposed to various genotoxic agents.