par Marcelis, Lionel 
;Van Overstraeten-Schlögel, Nancy;Lambermont, Javiera ;Bontems, Sébastien;Spinelli, Nicolas;Defrancq, Eric;Moucheron, Cécile ;Kirsch-De Mesmaeker, Andrée ;Raes, Martine
Référence ChemPlusChem, 79, 11, page (1597-1604)
Publication Publié, 2014
;Van Overstraeten-Schlögel, Nancy;Lambermont, Javiera ;Bontems, Sébastien;Spinelli, Nicolas;Defrancq, Eric;Moucheron, Cécile ;Kirsch-De Mesmaeker, Andrée ;Raes, MartineRéférence ChemPlusChem, 79, 11, page (1597-1604)
Publication Publié, 2014
Article révisé par les pairs
| Résumé : | A photoreactive ruthenium(II) complex that contains two tetraazaphenanthrene (TAP) and one phenanthroline (phen) ligands was synthesized and then tethered to (antisense) oligonucleotides (Ru-ASO) to target a destabilized GFP (dGFP). The specificity of the photoreaction of this Ru-ASO conjugate was studied in vitro by polyacrylamide gel electrophoresis (PAGE) experiments in denaturing conditions. Other nonspecific Ru-ASO conjugates were also prepared and evaluated with human keratinocytes that expressed dGFP. An illumination-dependent cytotoxicity was observed for most Ru-ASO conjugates that varied from 10 to almost 40%, but only the specific Ru-ASO conjugate was able to significantly reduce GFP expression in illuminated cells. |
