par Kupersztejn, Ruth 
;Thirion, Jacqueline
Référence Biochimica et biophysica acta, N. Nucleic acids and protein synthesis, 340, 3, page (314-322)
Publication Publié, 1974-03
;Thirion, Jacqueline Référence Biochimica et biophysica acta, N. Nucleic acids and protein synthesis, 340, 3, page (314-322)
Publication Publié, 1974-03
Article révisé par les pairs
| Résumé : | Crithidia luciliae has a single mitochondrion which occupies a large part of the cell. Two different types of ribosomes have been isolated from the cell: cytoplasmic 83 S and mitochondrial 60 S. The 83-S ribosomes are dissociated by Mg2+ at 10-3 mM into their subunits 62 S and 46 S. The 60-S ribosomes, obtained from the mitochondria, are easily dissociated into 45-S and 32-S subunits at higher concentrations of Mg2+ (3 mM). By these methods, an unusually large amount of mitochondrial ribosomes in Crithidias are found. The biosynthetic activity of the cytoplasmic and the mitochondrial ribosomes and their sensitivity to specific protein synthesis inhibitors (chloramphenicol, erythromycin, cycloheximide) has been studied in vitro. © 1974. |
