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The 5′ untranslated region of the At-P5R gene is involved in both transcriptional and post-transcriptional regulation

par Hua, Xue Jun;Van de Cotte, Brigitte;Van Montagu, Marc;Verbruggen, Nathalie
Référence Plant journal, 26, 2, page (157-169)
Publication Publié, 2001

Article révisé par les pairs

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Résumé :

The steady-state level of transcripts coding for the pyrroline-5-carboxylate reductase of Arabidopsis (At-P5R) increased under salt and heat stress, mainly because of an enhanced mRNA stability. However, the At-P5R protein level was not induced, and its translation was inhibited at initiation stage and probably also at later stages. Replacement of the 5′ untranslated region (5′UTR) and β-glucuronidase (gus) fusion analysis revealed that the first 92 bp region of the At-P5R 5′UTR was sufficient to mediate transcript stabilization and translation inhibition during salt and heat stresses. Furthermore, the first 92 bp region of the At-P5R 5′UTR was also involved in transcription efficiency in a promoter-dependent manner. The results demonstrated that the stress regulation of At-P5R is complex and involves the 5′UTR which acts at three levels, partly in opposing directions.