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Method for concentrating and purifying recombinant autonomous parvovirus vectors designed for tumour-cell-targeted gene therapy

par Avalosse, Bernard ;Dupont, Francis ;Spegelaere, Pierre ;Mine, Natacha ;Burny, Arsène
Référence Journal of virological methods, 62, 2, page (179-183)
Publication Publié, 1996-12

Article révisé par les pairs

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Résumé :

Recent work has highlighted the use of parvoviruses as potential vectors for tumour-cell-targeted gene therapy. The oncotropic properties of the prototype strain of minute virus of mice (MVMp) suggest that this virus might be a useful vehicle for introducing selectively therapeutic genes, e.g. lymphokine or suicide genes, into tumour cells and preferentially expressing them. But the low titre of recombinant virus stocks (105-106 infectious units per ml) and their high level of contamination by cell proteins make it practically impossible to evaluate their efficacy in in vivo systems. A technique is described for producing cellular contaminant-free stocks of recombinant virus particles, with titres up to 5 x 108 IU/ml.