par Robberecht, Patrick 
;Waelbroeck, Magali ;De Neef, Philippe ;Tastenoy, Michèle ;Gourlet, Philippe ;Cogniaux, Jacqueline;Christophe, Jean
Référence FEBS letters, 228, 2, page (251-355)
Publication Publié, 1988-02-15
;Waelbroeck, Magali ;De Neef, Philippe ;Tastenoy, Michèle ;Gourlet, Philippe ;Cogniaux, Jacqueline;Christophe, Jean Référence FEBS letters, 228, 2, page (251-355)
Publication Publié, 1988-02-15
Article révisé par les pairs
| Résumé : | A new type of VIP receptor was characterized in human SUP-T1 lymphoblasts. The order of potency of unlabeled peptides, in the presence of [125I]helodermin, was: helodermin(1-35)-NH2 = helodermin(1-27)-NH2 greater than helospectin greater than VIP = PHI greater than [D-Ser2]VIP greater than [D-Asp3]VIP greater than [D-His1]VIP greater than or equal to [D-Ala4]VIP greater than or equal to secretin = GRF. This specificity was distinct from that of all VIP receptors described so far in that: (i) the affinity for helodermin (Kd = 3 nM) was higher than that of VIP (Kd = 15 nM) and PHI (Kd = 20 nM); and (ii) position 4 played an important role in ligand binding. The labeled sites were likely to be functional receptors as adenylate cyclase in crude lymphoblastic membranes (200-10,000 x g pellets) was stimulated by peptides, in the presence of GTP, with the following order of potency: helodermin(1-35)-NH2 greater than helodermin(1-27)-NH2 greater than helospectin = VIP = PHI. |
