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Detection of Borrelia burgdorferi in biological samples using the polymerase chain reaction assay

par Debue, M.;Gautier, Philippe;Hackel, Christine;Van Elsen, Ary Maurice ;Herzog, Albert ;Bigaignon, Geoffroy;Bollen, Alex
Référence Research in microbiology, 142, 5, page (565-572)
Publication Publié, 1991

Article révisé par les pairs

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Résumé :

Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.