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Characterization and regulation of the expression of scyllatoxin (Leiurotoxin I) receptors in the human neuroblastoma cell line NB-OK 1

par Gossen, Denis ;Gesquiere, Jean Claude;Tastenoy, Michèle ;De Neef, Philippe ;Waelbroeck, Magali ;Christophe, Jean
Référence FEBS letters, 285, 2, page (271-274)
Publication Publié, 1991

Article révisé par les pairs

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Résumé :

125I-[Tyr2]scyllatoxin allowed to label a single class of high-affinity receptors in membranes from the human neuroblastoma cell line NB-OK 1. The K(d) of these receptors was 60 pM for scyllatoxin (Leiurotoxin I) and 20 pM for apamin and the B(max) was low (3.8 fmol/mg membrane protein). K+ increased toxin binding at low concentrations but exerted opposite effects at high concentrations. Ca2+, guanidinium and Na+ exerted only inhibitory effects on binding. Scyllatoxin binding sites were overexpressed 2.5-fold after a 24-h cell pretreatment with 2 mM butyrate. This effect was suppressed by cycloheximide.