par Chamart, Sylvie ;Hanocq, Michel ;Helson, Marianne;Devleeschouwer, Nadine ;Leclercq, Guy
Référence Journal of Chromatography - Biomedical Applications, 496, 2, page (365-375)
Publication Publié, 1989
Référence Journal of Chromatography - Biomedical Applications, 496, 2, page (365-375)
Publication Publié, 1989
Article révisé par les pairs
Résumé : | The 2-methyl derivatives of tamoxifen (2-methyltamoxifen and 2-methyl-4-hydroxytamoxifen) were extracted from a cell culture medium at pH 5.4 (Earle's Minimum Essential Medium) with an internal standard (tamoxifen) on a phenyl sorbent cartridge. The compounds were then separated by high-performance liquid chromatography on a nitrile column eluted with acetonitrile-methanol-0.05 M sodium dihydrogenphosphate (19.4:11.6:69, v/v) containing 0.11 nmol/1 disodium EDTA and determined by electrochemical detection at + 1.1 V vs.Ag/AgC1/3 M NaCl. The absolute detection limits were 50 pg for 2-methyl-4-hydroxytamoxifen and 100 pg for tamoxifen and 2-methyltamoxifen at a sensitivity of 1 nA/V. |