par Chamart, Sylvie ;Hanocq, Michel ;Leclercq, Guy ;Devleeschouwer, Nadine
Référence International journal of pharmaceutics, 99, 1, page (59-65)
Publication Publié, 1993
Article révisé par les pairs
Résumé : Adsorption of triphenylethylene compounds (tamoxifen, 4-hydroxytamoxifen and their 2-methyl derivatives) on glass and plastic surfaces was separately evaluated under specific conditions met during the preparation of solutions and their incubation. The composition of the culture medium (whether supplemented or not with serum), the material of the recipient (glass or plastic), the concentration of the drug and the substituents on the molecule dramatically affected the amount of active principle left in solution. When evaluated without cells, incubation of a less polar molecule (i.e., tamoxifen) at a low concentration (10-8 M) in a culture medium not supplemented with serum in a plastic dish left only 10% of the initial concentration after 1 h. Under the same conditions, the more polar 4-hydroxytamoxifen had a recovery rate of 20%. Advice is given on circumventing these problems which may lead to erroneous conclusions.