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Computational fluid dynamics design of a bioreactor mixing system

par Gelbgras, Valérie ;Drugmand, Jean-Christophe;Haut, Benoît
Référence WCCE-8(23 – 27 August 2009: Montréal, Canada), Proceedings of the 8th World Congress of Chemical Engineering
Publication Publié, 2009

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Résumé :

During a cell culture in a bioreactor, the cells are exposed to the shear stress generated by the culture medium mixing system. Beyond a limit shear stress, it induces the cell death. Thus, the shear stress is an important scale-up parameter. A better modeling of the generated shear stresses in the medium and their impact on the cell would achieve a bioreactor scale-up more effective. In this work, the shear stress is studied in a new design of mixing system especially developed by the biotechnological industry Artelis S.A., for a single-used animal cell bioreactor. The aims of this work are to develop a methodology to determine the shear stresses in a device, to model the effect of shear stresses on cellular viability and to use this model as optimization tool and to study the scale-up of the bioreactor. In a first step, the Artelis device is simulated by Computational Fluid Dynamics. These simulations are experimentally validated and are the base of the model of shear stresses generated in the device. In a second step, this model of shear stresses is confronted to the experimental measurements of cellular viabilities for different impeller rotation speed. Hence, a model of effects of shear stresses on the cellular viability is established. Finally, two scales-up are simulated to study their impacts on the cellular viability.