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Increased expression of macrophage migration inhibitory factor during progression to hypopharyngeal squamous cell carcinoma.

par Cludts, Stéphanie;Decaestecker, Christine ;Johnson, Bryon;Lechien, Jérôme;Leroy, Xavier;Kindt, Nadège;Kaltner, Herbert;André, Sabine;Gabius, Hans-Joachim;Saussez, Sven
Référence Anticancer research, 30, 9, page (3313-3319)
Publication Publié, 2010-09

Article révisé par les pairs

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Résumé :

BACKGROUND/AIM: To examine the presence of macrophage migration inhibitory factor (MIF) quantitatively in relation to neoplastic progression of hypopharyngeal squamous cell carcinoma (HSCC). MATERIALS AND METHODS: The presence of MIF was analysed by quantitative immunohistochemistry in sections of 81 HSCCs, and compared to 15 specimens of tumour-free epithelia (TF_E), 29 low-grade dysplasias (Low_D) and 25 high-grade dysplasias (High_D). In parallel, MIF expression was studied using Western blotting on a series of 19 fresh biopsies. RESULTS: A significant increase in MIF staining intensity (mean optical density) was observed in carcinoma samples compared to TF_E (p<10(-6)), Low_D (p=0.0006) or High_D (p=0.0006). Immunohistochemical MIF positivity was significantly higher in HSCCs than in TF_E (p=0.00001) or Low_D (p=0.001). The percentage of MIF-immunopositive cells (labelling index) significantly decreased in parallel with an apparent loss of histological differentiation (p=0.003). CONCLUSION: This study identified the presence of MIF as a parameter that positively correlates with neoplastic progression of HSCC and cell differentiation status.