par Charlier, N;Driesschaert, B;Wauthoz, Nathalie 
;Beghein, N;Préat, V;Amighi, Karim ;Marchand-Brynaert, J;Gallez, B.
Référence Journal of magnetic resonance, 197, 2, page (176-180)
Publication Publié, 2009-04
;Beghein, N;Préat, V;Amighi, Karim ;Marchand-Brynaert, J;Gallez, B.Référence Journal of magnetic resonance, 197, 2, page (176-180)
Publication Publié, 2009-04
Article révisé par les pairs
| Résumé : | This article reports the development and evaluation of two nano-emulsions (F45T-03/HFB and F15T-03/PFOB) containing fluorinated trityl radicals dissolved in perfluorocarbons. Preparation with a high-pressure homogenizer conferred sub-micronic size to both nano-emulsions. In vitro and in vivo EPR spectroscopy showed that the nano-emulsions had much greater oxygen sensitivity than the hydrophilic trityl, CT-03. In vivo experiments in rodents confirmed the ability of the nano-emulsions to follow the changes in oxygen concentration after induced ischemia. Histological evaluation of the tissue injected with the nano-emulsions revealed some acute toxicity for the F45T-03/HFB nano-emulsion but none for the F15T-03/PFOB nano-emulsion. These new formulations should be considered for further EPR oximetry experiments in pathophysiological situations where subtle changes in tissue oxygenation are expected. |
