par Lebrun, Philippe ;Malaisse, Willy ;Herchuelz, André
Référence Biochemical pharmacology, 30, 24, page (3291-3294)
Publication Publié, 1981-12
Article révisé par les pairs
Résumé : Verapamil and the ion cobalt, both considered as calcium antagonists, provoked a rapid, dose-related, and rapidly reversible inhibition of 86Rb outflow from prelabelled pancreatic islets. The decrease in 86Rb outflow was not affected by the concentration of extracellular calcium, identical results being obtained at normal (1 mM) and high calcium concentration (5 mM), as well as in the absence of calcium and presence of EGTA. The capacity of glucose in high concentration (11.1-27.2 mM) to inhibit 86Rb outflow was considerably decreased in islets exposed to either verapamil or cobalt. These findings suggest that verapamil and cobalt may not be adequate tools to distinguish between changes in K+ conductance and calcium inflow, respectively, as determinants of the bioelectrical response of the pancreatic β-cell to glucose.