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The cloning and sequencing of cervine interleukin 10

par Lockhart, Euan A.;Slobbe, Lynn J.;Droogmans, Louis ;Griffin, J. Frank T.;Buchan, Glenn S.
Référence DNA Sequence - Journal of DNA Sequencing and Mapping, 5, page (265-268)
Publication Publié, 1995

Article révisé par les pairs

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Résumé :

We report the cloning and sequencing of the cervine interleukin-10 gene. Specific cDNA was amplified by PCR using primers based on the bovine sequence. This was cloned into pGEM 5Zf and several clones were sequenced. The 762 nucleotide product coded for a 179 amino acid protein which was 86% homologous with its bovine and 77% homologous with its human counterparts. There is a strongly hydrophobic signal sequence consisting of the first 20 amino acids and a potential glycosylation site at amino acids 134-136. There are three regions, comprising 34% of the protein, which show complete homology between the cervine, bovine and human sequences. The transcription of the gene was shown by Northern Blotting where a single, 1.8Kb, mRNA transcript was detected 4-8 hours after activation of peripheral blood mononuclear cells with mitogen. © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.