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Restriction mapping of synthetic thyroglobulin structural gene as a means of investigating thyroglobulin structure.

par Vassart, Gilbert ;Brocas, Huguette
Référence Biochimica et biophysica acta, 610, 1, page (189-194)
Publication Publié, 1980-11

Article révisé par les pairs

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Résumé :

Bovine 33 S thyroglobulin mRNA was reverse transcribed into double-stranded DNA under conditions allowing the synthesis of a complete 8 kilobase pair copy. A physical map of the resulting synthetic thyroglobulin structural gene was constructed using six restriction endonucleases. The following conclusions could be drawn: (i) the polypeptide chains in thyroglobulin subunits are identical; (ii) thyroglobulin is composed of a major class of molecules sharing the same primary structure; (iii) there is no evidence for precise internal repetition in the structure of thyroglobulin subunits.