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Metabolism of the dimethyl ester of [2,3-(13)C]succinic acid in rat hepatocytes.

par Malaisse, Willy ;Ladrière, Laurence ;Jijakli, Hassan ;Laatikainen, R;Niemitz, M;Verbruggen, Ingrid;Biesernans, M;Willem, Rudolph
Référence Molecular and cellular biochemistry, 189, 1-2, page (137-144)
Publication Publié, 1998-12

Article révisé par les pairs

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Résumé :

Hepatocytes prepared from overnight fasted rats were incubated for 120 min in the presence of the dimethyl ester of [2,3-(13)C]succinic acid (10 mM). The identification and quantification of 13C-enriched metabolites in the incubation medium were performed by a novel computational strategy for the deconvolution of NMR spectra with multiplet structures and constraints. The generation of 13C-labelled metabolites, including succinate, fumarate, malate, lactate, alanine, aspartate and glucose accounted for about half of the initial amount of the ester present in the incubation medium. A fair correlation was observed between the experimental abundance of each 13C-labelled glucose isotopomer and the corresponding values derived from a model for the metabolism of [2,3-(13)C]succinate. Newly formed glucose was more efficiently labelled in the carbon C5 than C2, as well as the carbon C6 than C1, supporting the concept that D-glyceraldehyde-3-phosphate may undergo enzyme-to-enzyme channelling between glyceraldehyde-3-phosphate dehydrogenase and phosphofructoaldolase.