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The adjuvant monophosphoryl lipid A increases the function of antigen-presenting cells.

par De Becker, Geneviève ;Moulin, V;Pajak, Bernard ;Bruck, C;Francotte, Myriam;Thiriart, Clotilde;Urbain, Jacques ;Moser, Muriel
Référence International immunology, 12, 6, page (807-815)
Publication Publié, 2000-06

Article révisé par les pairs

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Résumé :

The induction of immune responses in vivo is typically performed with antigens administered in external adjuvants, like alum, complete Freund's adjuvant, LPS and, more recently, monophosphoryl lipid A (MPL). However, the role of the adjuvant is still poorly defined. The aim of this study was to test whether the MPL affects the function of antigen-presenting cells (APC) in vitro and in vivo. Antigen-pulsed APC [including macrophages, B cells and dendritic cells (DC)] were incubated or not with MPL, and their ability to sensitize naive T cells was tested in vitro and in vivo. The data show that MPL enhances the ability of macrophages and B cells to sensitize naive T cells, and confers to them the capacity to induce the development of T(h)1 and T(h)2. Administration of MPL i.v. in mice results in the redistribution of fully mature DC in the T cell area of the spleen. These observations suggest that MPL may induce an antigen-specific primary immune response by provoking the migration and maturation of DC that are the physiological adjuvant of the immune system.