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DiC14-amidine cationic liposomes stimulate myeloid dendritic cells through Toll-like receptor 4.

par Tanaka, Tetsuya;Legat, Amandine ;Adam, Emmanuelle ;Steuve, Jonathan ;Gatot, Jean-Stéphane ;Vandenbranden, Michel ;Ulianov, Liliana;Lonez, Caroline ;Ruysschaert, Jean Marie ;Muraille, Eric ;Tuynder, Marcel;Goldman, Michel ;Jacquet, Alain
Référence European Journal of Immunology, 38, 5, page (1351-1357)
Publication Publié, 2008-05

Article révisé par les pairs

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Résumé :

DiC14-amidine cationic liposomes were recently shown to promote Th1 responses when mixed with allergen. To further define the mode of action of diC14-amidine as potential vaccine adjuvant, we characterized its effects on mouse and human myeloid dendritic cells (DC). First, we observed that, as compared with two other cationic liposomes, only diC14-amidine liposomes induced the production of IL-12p40 and TNF-alpha by mouse bone marrow-derived DC. DiC14-amidine liposomes also activated human DC, as shown by synthesis of IL-12p40 and TNF-alpha, accumulation of IL-6, IFN-beta and CXCL10 mRNA, and up-regulation of membrane expression of CD80 and CD86. DC stimulation by diC14-amidine liposomes was associated with activation of NF-kappaB, ERK1/2, JNK and p38 MAP kinases. Finally, we demonstrated in mouse and human cells that diC14-amidine liposomes use Toll-like receptor 4 to elicit both MyD88-dependent and Toll/IL-1R-containing adaptor inducing interferon IFN-beta (TRIF)-dependent responses.