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Optimal kinetics for quantification of antigen-induced cytokines in human peripheral blood mononuclear cells by real-time PCR and by ELISA.

par Listvanova, Slavka;Temmerman, Stéphane ;Stordeur, Patrick ;Verscheure, Virginie ;Place, Sammy ;Zhou, Ling ;Locht, Camille ;Mascart, Françoise
Référence Journal of immunological methods, 281, 1-2, page (27-35)
Publication Publié, 2003-10

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Titre:	Optimal kinetics for quantification of antigen-induced cytokines in human peripheral blood mononuclear cells by real-time PCR and by ELISA.
Auteur:	Listvanova, Slavka; Temmerman, Stéphane; Stordeur, Patrick; Verscheure, Virginie; Place, Sammy; Zhou, Ling; Locht, Camille; Mascart, Françoise
Informations sur la publication:	Journal of immunological methods, 281, 1-2, page (27-35)
Statut de publication:	Publié, 2003-10
Sujet CREF:	Sciences bio-médicales et agricoles
Mots-clés:	Heparin-binding haemagglutinin
	Interferon-gamma
	Interleukin-13
	Purified protein derivative
	Real-time PCR
	Tuberculosis
MeSH keywords:	Antigens -- immunology
	Cytokines -- biosynthesis
	Cytokines -- genetics
	Enzyme-Linked Immunosorbent Assay -- methods
	Gene Dosage
	Hemagglutinins -- immunology
	Humans
	Kinetics
	Lectins
	Leukocytes, Mononuclear -- immunology
	Polymerase Chain Reaction -- methods
	RNA, Messenger -- analysis
	Tuberculin -- immunology
Note générale:	Journal Article
	Research Support, Non-U.S. Gov't
Langue:	Anglais
Identificateurs:	urn:issn:0022-1759
	info:doi/10.1016/S0022-1759(03)00267-9
	info:pii/S0022175903002679
	info:scp/0142216542
	info:pmid/14580879