par Wouters, Johan 
;Tricot, Catherine ;Durbecq, Virginie ;Roovers, Martine ;Stalon, Victor ;Droogmans, Louis
Référence Acta crystallographica. Section D, Biological crystallography, 59, 1, page (152-154)
Publication Publié, 2003-01
;Tricot, Catherine ;Durbecq, Virginie ;Roovers, Martine ;Stalon, Victor ;Droogmans, Louis Référence Acta crystallographica. Section D, Biological crystallography, 59, 1, page (152-154)
Publication Publié, 2003-01
Article révisé par les pairs
| Résumé : | Thermotoga maritima TruB, an enzyme responsible for the formation of pseudouridine in tRNA, has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li(2)SO(4), 20% glycerol, 13% PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 47.39, b = 83.88, c = 98.72 A, and diffract to 2.0 A resolution using synchrotron radiation. A solution was obtained by molecular replacement using part of the recently published crystal structure of Escherichia coli TruB bound to a synthetic RNA. |
