Résumé : Objective: There is growing evidence of the benefits of mindfulness practices for workers’ well-being. However, most studies have focused primarily on hedonic well-being, rather than considering both its hedonic and eudaimonic aspects. Furthermore, the specific mechanisms underlying the effects of mindfulness on workers’ well-being remain understudied. This study examined two potential mediators in the relationship between mindfulness and workers’ well-being: cognitive appraisal (i.e., threat and challenge appraisal) and cognitive decentring. Method: It was hypothesised that mindfulness training would enhance workers’ well-being by increasing decentring (the first mediator), which would, in turn, predict changes in cognitive appraisal (the second mediator). This hypothesis was tested using a quasi-experimental design comparing the effects of an eight-week Mindfulness-Based Stress Reduction (MBSR) programme (n = 31) with those of a waitlist control group (n = 27). Results: The intervention improved workers’ well-being, trait mindfulness, decentring, and threat appraisal. No significant effect was found for challenge appraisal. Mindfulness predicted approximately 66% of the variance in workers’ well-being, and increased well-being was mediated by greater decentring, which in turn contributed to a reduction in threat appraisal. Conclusion: Mindfulness training enhanced workers’ mental well-being by increasing their capacity to distance themselves from stimuli, thereby reducing their tendency to appraise such stimuli as threatening.