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Protocol to obtain long movement trajectories of leaders and followers in ant tandem runs

par Tanaka, Yasunari;Valentini, Gabriele ;Pratt, Stephen;Shimoji, Hiroyuki;Mizumoto, Nobuaki
Référence STAR Protocols, 4, 4, 102769
Publication Publié, 2023-12

Article révisé par les pairs

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Résumé :

Tandem running in ants is a sophisticated form of communication. Precise measurement of movement coordination by the tandem pair can shed light on social interactions. Here, we describe an integrative approach to obtain long movement trajectories of a specific tandem pair within a crowd of ants. We describe a maze-like arena, video recording and editing, and movement tracking. We integrate two pieces of image-based tracking software that have distinct individual assignment strategies. This protocol aids comparative studies of recruitment communication across species. For complete details on the use and execution of this protocol, please refer to Mizumoto et al. (2023)1 and Valentini et al. (2020).2