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A systematic elementary flux mode selection procedure for deriving macroscopic bioreaction models from metabolic networks

par Maton, M.;Bogaerts, Philippe ;Wouwer, Alain Vande
Référence Journal of process control, 118, page (170-184)
Publication Publié, 2022-10-01

Article révisé par les pairs

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Résumé :

Elementary flux modes (EFMs) are an important concept in metabolic pathway analysis and for the derivation of macroscopic dynamic models. However, the computation of elementary flux vectors is facing combinatorial explosion with the size of the metabolic network, which hinders widespread application. This study proposes a systematic elementary flux mode reduction procedure to derive reduced-order dynamic models starting from an initial set of EFMs either generated by complete enumeration or subset selection. The procedure proceeds in several steps, including geometric and optimization-based criteria. The methodology ends up with a macroscopic bioreaction scheme with a reaction number smaller than that of the measured species, and shows very satisfactory prediction results, as illustrated with data of batch cultures of CHO-320 cells.