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Rapid de- and Regeneration of The Zebrafish Olfactory System

par Kocagöz, Yigit;Eski, Sema Elif ;Fuss, Stefan H.
Référence International Congress of the Molecular Biology Association of Turkey (6th: 2018-09-05: Izmir, Turkey)
Publication Publié, 2018-09-05

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Résumé :

The peripheral olfactory epithelium (OE) has an exceptionallyhigh regenerative capacity. We use an experimental injury model inzebrafish to study the cellular responses underlying OE regeneration.Nasal irrigation with the detergent Triton X-100 results in the loss ofup to 80% of the olfactory sensory neuron population within 24 h andnear complete recovery within 7 d. We use BrdU incorporation assaysand expression analysis of cell type-specific markers to describethe dynamics of the regeneration response and to identify relevantprogenitor populations contributing to OE regeneration. We identified abasally located cell population, which expresses the stem cell markerssox2, krt5, and tp63, which shows increased neurogenic activityupon injury and is distinct from cells that contribute to maintenanceneurogenesis. Using transcriptome analysis over the time course of de- and regeneration, we have identified candidate molecular pathwaysthat may be critical for the selective activation of repair neurogenesis.Supported by TUBİTAK 113T038.