• Citer

Defining appropriate parameters for robotic subretinal drug delivery ensures minimal reflux

par Ladha, Reza ;Smit, Jorrit;Cerfonteijn, Esmée;Meenink, Thijs;de Smet, Marc D
Référence Euretina Meeting 2021 (September 2021: Amsterdam, The Netherlands)
Publication Non publié, 2021-09

Poster de conférence

• Détails
• Contenu

Résumé :

Purpose: Vitreous reflux during subretinal injection limits the degree and surface area transfected and is wasteful of a precious product. The precision and stability offered by the Preceyes platform allows for an optimized, time-independent delivery. The purpose of this study is to identify parameters important to insure reflux-free robotic subretinal drug delivery.Setting/Venue: Comparative trial in an ex vivo animal modelMethods: Freshly harvested porcine eyes underwent a standard pars plana vitrectomy with IOP control under microscopic visualization through a Rescan 700 Zeiss device; followed by subretinal injection using a 41G Teflon needle, with the Preceyes Surgical System (PSS). The following subretinal injection cannula parameters were analyzed: speed of injection, tip configuration, and positioningResults: Following multiple injections, positioning of the tip above the RPE without penetration through Bruch’s membrane was possible in all cases by using a stepwise penetration algorithm irrespective of the angle of approach. Blunt needles did not penetrate as well as needle tips with polished edges. Small robotically controlled rotations and wiggling of the injection cannula did not result in a cleaner penetration or less reflux. Sufficient delay is required between the completion of the injection and retraction of the needle to prevent reflux.Conclusions: Robotics allows for a better understanding of the injection process and a determination of critical parameters and steps. Several parameters appear critical to an efficient reflux-freeinjection.Theme: New Drug Treatment and Technology