par America, Michelle 
;Bostaille, Naguissa ;Eubelen, Marie ;Martin, Maud ;Stainier, Didier Y.R.;Vanhollebeke, Benoît
Référence Cell reports, 39, 9, page (110902)
Publication Publié, 2022-05-01
;Bostaille, Naguissa ;Eubelen, Marie ;Martin, Maud ;Stainier, Didier Y.R.;Vanhollebeke, Benoît Référence Cell reports, 39, 9, page (110902)
Publication Publié, 2022-05-01
Article révisé par les pairs
| Résumé : | Within the central nervous system, Wnt7a/b are unambiguously discriminated from other Wnt ligands by an endothelial receptor complex made of the glycosylphosphatidylinositol (GPI)-anchored Reck and the adhesion G protein-coupled receptor (GPCR) Gpr124. Reck is a Wnt7a/b-specific receptor, while Gpr124 facilitates the delivery of Reck-bound Wnt7a/b ligands to Frizzled, through partially characterized mechanisms. We report that, in zebrafish, the Gpr124-Frizzled interactions are dominated by intracellular scaffolds that exploit the striking molecular mimicry between Gpr124 and Frizzled intracellular domains (ICDs): an internal Dvl-binding motif and a C-terminal ETTV motif that recruits Dlg4 and Magi3. By contrast, mammalian Gpr124 receptors exhibit an ICD-independent interaction mechanism governed by species-specific attributes of their transmembrane and extracellular domains. This mechanism seemingly evolved to replace the Dvl-mediated mechanism. By contrasting zebrafish, mouse, and human Gpr124, this study provides insights into the evolution of Gpr124/Reck function across the vertebrate clade, a receptor complex uniquely implicated in Wnt ligand-specific cellular responses. |
