par Frost, Hannah R C H.;Davies, Mark R;Velusamy, Srinivasan;Delforge, Valérie ;Erhart, Annette;Darboe, Saffiatou;Steer, Andrew C;Walker, Mark J;Beall, Bernard W;Botteaux, Anne ;Smeesters, Pierre
Référence Clinical microbiology and infection, 26, 7, page (946.e5-946.e8)
Publication Publié, 2020-07-01
Référence Clinical microbiology and infection, 26, 7, page (946.e5-946.e8)
Publication Publié, 2020-07-01
Article révisé par les pairs
Résumé : | PCR-based typing of the emm gene Streptococcus pyogenes often results in the amplification of multiple bands. This has resulted in the misclassification of strains into types based on non-emm gene sequences. We aimed to improve the specificity of the emm typing PCR reaction using a primer called CDC3, the sequence for which has been previously used to identify emm genes in silico. |