Article révisé par les pairs
Résumé : A macroscopic model that takes into account phenomena of overflow metabolism within glycolysis and glutaminolysis is proposed to simulate hybridoma HB-58 cell cultures. The model of central carbon metabolism is reduced to a set of macroscopic reactions. The macroscopic model describes three metabolism states: respiratory metabolism, overflow metabolism and critical metabolism. It is validated with experimental data of fed-batch hybridoma cultures and successfully predicts the dynamics of cell growth and death, substrate consumption (glutamine and glucose) and metabolites production (lactate and ammonia). Model parameters and confidence intervals are obtained via a non linear least squares identification. This model, on the one hand, allows quantitatively describing overflow metabolism in mammalian cell cultures and, one the other hand, will be valuable for monitoring and control of fed-batch cultures in order to optimize the process.