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A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability Upon Metabolic Stress.

par Denanglaire, Sébastien ;Pirnay, Tiphene ;Leo, Oberdan ;Andris, Fabienne
Référence Methods in molecular biology, 1732, page (465-476)
Publication Publié, 2018

Article révisé par les pairs

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Résumé :

Distinct lymphocyte subpopulations display discrete metabolic profiles and are differently affected by metabolic resource variations, making the analysis of lymphocyte survival in a complex tissue in response to metabolic stress highly challenging. Here we describe a flow cytometry-based method allowing simultaneous cell identification and viable cell counting in mixed lymphocyte populations without extensive cell subset purification procedures. The example provided herein illustrates the role of AMPK in T lymphocyte survival in response to the mitochondrial poison oligomycin.