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The molecular mechanism of induced mutations and an in vitro biochemical assay for mutagenesis

par Radman, Miroslaw ;Caillet Fauquet, Perrine ;Defais, Martine ;Villani, Giuseppe
Référence IARC (International Agency for Research on Cancer) Scientific Publications, 12, page (537-548)
Publication Publié, 1976

Article révisé par les pairs

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Résumé :

Induction of a hypothetical error prone DNA polymerase activity following mutagenic treatments has recently been proposed to explain survival and mutagenesis in Escherichia coli and its viruses. The authors summarize much of their largely yet unpublished results obtained with E. coli and its viruses λ and phiX174, whose genomes are a double stranded and a single stranded DNA, respectively, following ultraviolet (UV) irradiation. These bacteriophages provide in vivo substrates to test for the repair and mutagenic cellular activities independently of cell death. The authors review evidence showing that the results obtained with UV irradiation are relevant to chemical mutagenesis as well. Their studies led to what is the first genuine in vitro biochemical assay for mutagenesis.