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Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress.

par Delatte, Benjamin ;Jeschke, Jana ;Defrance, Matthieu ;Bachman, Martin;Creppe, Catherine ;Calonne, Emilie ;Bizet, Martin ;Deplus, Rachel ;Marroquí, Laura ;Libin, Myriam ;Ravichandran, Mirunalini;Mascart, Françoise ;Eizirik, Decio L. ;Murrell, Adele;Jurkowski, Tomasz Piotr;Fuks, François
Référence Scientific reports, 5, page (12714)
Publication Publié, 2015-08

Article révisé par les pairs

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Résumé :

The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.