par Van Eycke, Yves-Remi ;Allard, Justine ;Derock, Mélanie ;Salmon, Isabelle ;Debeir, Olivier ;Decaestecker, Christine
Référence (13-16 April 2016: Prague, Czech Republic), 2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI), IEEE, page (795 - 798)
Publication Publié, 2016-04
Publication dans des actes
Résumé : We propose to adapt to immunohistochemistry (IHC) some methods proposed to normalize images from histological slices stained with hematoxylin-eosin (H&E). Our final aim is to provide a coherent quantitative characterization of IHC biomarkers across different IHC batches with possible staining variations. In contrast to H&E, IHC staining strongly varies with the tissue analyzed and the protein targeted, making image normalization challenging. To solve this problem, we added in each IHC batch a slice from a reference tissue microarray (TMA) and then digitalized it to establish an inter-batch normalization transform. A comparison of two methods adapted to the specificity of IHC-stained slides evidences some normalization requirements to make valid IHC biomarker quantification across different staining batches.