par Lebrun, Philippe ;Malaisse, Willy ;Herchuelz, André
Référence American journal of physiology: endocrinology and metabolism, 6, 3, page (E196-E205)
Publication Publié, 1982
Article révisé par les pairs
Résumé : Severa insulin secretagogues increase 45Ca efflux from islets prelabeled with the tracer in the presence of glucose, an effect attributable to stimulation of 40Ca entry into islet cells. When the islets are prelabeled in the absence instead of presence of glucose, the 45Ca becomes more readily releasable, and the secretory response to nutrient or ionic secretagogues is decreased. Such a decrease is more marked in response to glucose than other secretagogues because the prior nutrient deprivation also impairs the metabolism of glucose in the islet cells. In the islets prelabeled in the absence of glucose, both glyceraldehyde and 2-ketoisocaproate dramatically stimulate 45Ca efflux. This effect persists in the absence of extracellular Ca2+ or presence of the calmodulin-antagonist trifluoperazine. It may correspond to a nutrient-induced intracellular Ca2+ movement, the existence of which would be unmasked in islets prelabeled in the absence of glucose. This so-far-undetected Ca2+ movement may play a role in the secretory response to nutrient secretagogues.