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Les groupes actifs de la ribonucléase. I. Action du parachloromercuribenzoate de sodium sur le système ARN-RNase

par Ledoux, Lucien
Référence Biochimica et biophysica acta, 11, C, page (517-523)
Publication Publié, 1953

Article révisé par les pairs

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Résumé :

Sodium p-chloromercuribenzoate affects the action of ribonuclease by causing, depending on experimental conditions, either an inhibition or an activation. 1. 1. Inhibition results from a real interaction between PCMB and ribonuclease; it is dependent on pH, on relative concentrations and on time of incubation. It is reversible (i.e. under the action of OH- ions and glutathione) and probably corresponds to an inactivation of functional -SH groups 2. 2. Activation is due to an interaction of ribonucleic acid with PCMB favouring enzymic activity. 3. 3. A study of spectral variations of the nucleotides and nitrogen bases under the action of PCMB suggests that this reagent is active on polarisable C = O groups of these substances. Electrometric measurements give further evidence for this interpretation. © 1953.