par Lejeune-Lenain, C. ;Kina, S.;Bosson, Danièle
Référence Chromatographia, 24, 1, page (333-338)
Publication Publié, 1987-12
Article révisé par les pairs
Résumé : A method has been developed to measure simultaneously in human plasma the mineralocorticoids and glucocorticoids involved in mineralocorticoid excess. Briefly: plasma extracts were chromatographied in a fully-automated procedure on a lichrospher-diol column using a hexane: isoproanol: trithylamine gradient. Eluates were collected on a preprogrammed fraction collector. Final quantification was achieved by RIA on the selected fractions. This procedure allowed: 1) the separation of each measured steroid from plasma contaminants; 2) precise collection; 3) quantification of mineralocorticoids with a precision and sensitivity equivalent to that of direct methods; and 4) improved specificity. © 1987 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.