par Srinivasan, P.R.;Faures, Anne ;Errera, Maurice Leo
Référence Biochimica et biophysica acta, 65, 3, page (501-505)
Publication Publié, 1962-12
Article révisé par les pairs
Résumé : Three methods of isolation of nuclei of HeLa cells were investigated in connection with studies of pulse-labeled RNA in HeLa cells: (a) sucrose-homogenization, (b) osmotic shock, (c)_citric acid. Of the three methods only the nuclei isolated by citric acid method conserved all the radioactivity of the pulse-labeled RNA found in the nuclei of whole cells. Isolation by the other two methods resulted in a 20 and 17% loss of the labeled RNA of the nuclei. © 1962.