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A simple and rapid protocol to non-enzymatically dissociate fresh human tissues for the analysis of infiltrating lymphocytes

par Garaud, Soizic ;Gu-Trantien, Chunyan ;Lodewyckx, Jean Nicolas;Boisson, Anaïs;De Silva, Pushpamali;Buisseret, Laurence ;Migliori, Edoardo;Libin, Myriam ;Naveaux, Céline;Duvillier, Hugues ;Willard-Gallo, Karen
Référence Journal of Visualized Experiments, 94, e52392
Publication Publié, 2014-12

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Titre:	A simple and rapid protocol to non-enzymatically dissociate fresh human tissues for the analysis of infiltrating lymphocytes
Auteur:	Garaud, Soizic; Gu-Trantien, Chunyan; Lodewyckx, Jean Nicolas; Boisson, Anaïs; De Silva, Pushpamali; Buisseret, Laurence; Migliori, Edoardo; Libin, Myriam; Naveaux, Céline; Duvillier, Hugues; Willard-Gallo, Karen
Informations sur la publication:	Journal of Visualized Experiments, 94, e52392
Statut de publication:	Publié, 2014-12
Sujet CREF:	Neurosciences cognitives
	Génie chimique
	Microbiologie et protistologie [bacteriol.,virolog.,mycolog.]
	Immunologie
	Sciences bio-médicales et agricoles
Mots-clés:	Breast cancer
	CD45+
	Fresh tissue homogenate
	Immunology
	Issue 94
	Non-enzymatic dissociation
	Primary tissue supernatant
	Tumor immunology
	Tumor infiltrating lymphocytes
MeSH keywords:	Antigens, CD45 -- chemistry
	Breast -- cytology -- immunology
	Breast Neoplasms -- immunology -- pathology
	Cytological Techniques -- methods
	Female
	Humans
	Lymphocytes, Tumor-Infiltrating -- cytology -- immunology -- pathology
Note générale:	SCOPUS: ar.j
	SCOPUS: ar.j
Langue:	Anglais
Identificateurs:	urn:issn:1940-087X
	info:doi/10.3791/52392
	info:scp/84917695112
	info:pmid/25548995
	PMC4396968