par Borst, Piet;Fase-Fowler, Francesca;Steinert, Maurice ;Van Assel, Suzanne
Référence Experimental cell research, 110, 1, page (167-173)
Publication Publié, 1977
Article révisé par les pairs
Résumé : We have analysed the kinetoplast DNA (kDNA) of Trypanosoma mega for the presence of DNA circles other than mini-circles and mini-circle oligomers. Degradation of networks with restriction endonucleases EcoRI, HhaI or PstI or S1 nuclease yields minor bands on gels, in addition to the mini-circle bands. The combined molecular weight of the minor bands is 15-17 × 106. Electron micrographs of kinetoplast DNA spread in a protein monolayer contain occasional circles longer than 3 μm. The majority class of these circles has a contour length corresponding to a mass of 16.1 × 106 D. We conclude that these maxi-circles, catenated into mini-circle networks, are an essential component of intact kinetoplast DNA of Trypanosoma mega. © 1977.