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Chapter 7 Codon Recognition: Evaluation of the Effects of Modified Bases in the Anticodon Loop of Trna Using the Temperature-Jump Relaxation Method

par Grosjean, Henri ;Houssier, Claude
Référence Journal of chromatography library, 45, PART A, page (A255-A295)
Publication Publié, 1990

Article révisé par les pairs

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Résumé :

This chapter describes the temperature-jump (T-jump) technique and its uses in the study of two tRNA molecules having complementary or quasi-complementary anticodons. This approach offers a sensitive means for accurately detecting event in tiny differences in the interaction energies between the two tRNAs. The chapter emphasizes on the effect of various (hyper)-modifications found at positions 34 and 37 of the anticodon on the thermodynamic and kinetic parameters, as well as on the spectral properties of the duplexes. The results enlighten the importance of these bases in the stabilization of the tRNA association. As the anticodon–anticodon interactions can mimic the codon-anticodon association process on the ribosome, such studies allow insights into some of the structural and dynamic parameters of tRNA, intrinsic to the anticodon loop and stem that are essential for the efficiency and accuracy of the whole translation process. © 1990, Elsevier Inc. All rights reserved.