par Henquin, Jean Claude;Malvaux, Paul;Lambert, André 
Référence Diabetologia, 10, 1, page (61-68)
Publication Publié, 1974-02
Référence Diabetologia, 10, 1, page (61-68)
Publication Publié, 1974-02
Article révisé par les pairs
| Résumé : | The use of polyethylene glycol 6000 to separate free and antibody-bound ligand has been applied to the radioimmunoassay of glucagon. Equalization of protein content in all tubes before precipitation of the glucagon-antibody complex was required. Time between addition of the polymer and centrifugation had no detectable effect. Degradation of 131I-glucagon during incubation was best prevented by a combination of benzamidine (5mM) and Trasylol® (500KIE/tube). Sensitivity of the assay permitted discrimination of buffer or plasma samples (100 μl) whose glucagon contents differed from 25 pg/ml, under 100 pg/ml, and 35 pg/ml, under 200 pg/ml. Reproducibility was 9.5% (coefficient of variation) for plasmas with glucagon concentrations ranging from 100 to 300 pg/ml. Recovery of exogenous glucagon added to plasma was satisfactory. Measurement of glucagon was possible in fasting plasma samples diluted up to 1/8. The separation method described appears to be easy and reliable, especially when large numbers of samples are routinely handled. © 1974 Springer-Verlag. |
